Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.2K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a Core Outcome Domain Set for Facial Aging.

JAMA dermatology·2026
Same author

HSR26-247: Real-World Quality of Life in Patients Treated With Cemiplimab for Advanced Cutaneous Squamous Cell Carcinoma: Results of CemiplimAb Survivorship Epidemiology (CASE) Study.

Journal of the National Comprehensive Cancer Network : JNCCN·2026
Same author

HSR26-247: Real-World Quality of Life in Patients Treated With Cemiplimab for Advanced Cutaneous Squamous Cell Carcinoma: Results of CemiplimAb Survivorship Epidemiology (CASE) Study.

Journal of the National Comprehensive Cancer Network : JNCCN·2026
Same author

Vacuum-based specimen holder for rapid surgical specimen imaging.

Biomedical optics express·2026
Same author

Two-photon microscopy allows for rapid imaging and diagnosis of Mohs specimens.

JAAD case reports·2025
Same author

High area and volumetric throughput parallel two-photon fluorescence microscopy using silicon photomultiplier arrays.

Photonics research·2025

Related Experiment Video

Updated: Jan 14, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

8.4K

High-throughput hyperspectral fluorescence imaging using a high-speed silicon photomultiplier array.

Chi Z Huang1, Vincent D Ching-Roa1, Connor M Heckman1

  • 1Department of Biomedical Engineering, University of Rochester, 207 Goergen Hall, Box 270168, Rochester, NY 14627, USA.

Biomedical Optics Express
|October 20, 2025
PubMed
Summary

This study introduces a low-cost hyperspectral detection system using silicon photomultiplier arrays for high-speed multiplex fluorescence imaging. The system achieves high spectral resolution and throughput, overcoming previous limitations in sensitivity and cost.

More Related Videos

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

12.4K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.1K

Related Experiment Videos

Last Updated: Jan 14, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

8.4K
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

12.4K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.1K

Area of Science:

  • Biophotonics
  • Microscopy
  • Spectroscopy

Background:

  • High-speed multiplex imaging of fluorescent probes faces limitations in spectral resolution, sensitivity, cost, and detector light throughput.
  • Existing systems struggle to efficiently separate and detect spectrally overlapping fluorophores.

Purpose of the Study:

  • To develop a cost-effective hyperspectral detection system for high-speed multiplex fluorescence imaging.
  • To overcome the limitations of current systems by enhancing spectral resolution and light throughput.

Main Methods:

  • Implementation of a hyperspectral detection system utilizing a silicon photomultiplier array.
  • Integration with a conventional two-photon microscope.
  • Application of a generalized spectral unmixing model for fluorophore separation.

Main Results:

  • Demonstration of 16 spectral channel imaging at 50 MP/s (800M spectra/second).
  • Successful extraction of spectrally overlapping fluorophores.
  • Enabled multiplexing of multiple contrast agents and detection of subtle spectral shifts.

Conclusions:

  • Silicon photomultiplier arrays offer a promising, low-cost solution for high-speed, high-throughput hyperspectral imaging.
  • The developed system significantly advances multiplex fluorescence imaging capabilities.
  • Potential for broad application in various biological and chemical sensing scenarios.