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Poloxamer-based thermosensitive injectable hydrogels containing a self-assembling peptide for In situ gelation.

S Sana Sayedipour1,2, Timo Schomann1,3, Sanne M van de Looij4

  • 1Department of Radiology, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.

Computational and Structural Biotechnology Journal
|October 20, 2025
PubMed
Summary

This study developed a novel injectable hydrogel by combining poloxamer 407 (P407) with a self-assembling peptide. This enhanced hydrogel offers improved mechanical strength and rapid gelation for osteoarthritis (OA) treatment.

Keywords:
In situ gelationPoloxamerSelf-assembling peptideThermosensitive hydrogel

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Drug Delivery Systems

Background:

  • Poloxamer 407 (P407) hydrogels are widely used but often lack sufficient mechanical strength for therapeutic applications.
  • Enhancing the properties of injectable hydrogels is crucial for effective site-specific drug delivery and tissue regeneration.

Purpose of the Study:

  • To develop and characterize a novel thermosensitive injectable hydrogel by incorporating a self-assembling peptide into P407.
  • To improve the biomechanical properties, gelation kinetics, and therapeutic cargo release of P407 for intra-articular (i.a.) applications.

Main Methods:

  • Formulation of a P407-peptide hybrid hydrogel.
  • Assessment of gelation time and rheological properties (storage modulus).
  • In vitro biocompatibility testing using human chondrocytes (MTS assay, LIVE/DEAD staining).
  • In vivo evaluation in an osteoarthritis mouse model using near-infrared fluorescent (NIRF) dye tracking.

Main Results:

  • The P407-peptide hydrogel exhibited significantly faster gelation compared to P407 alone.
  • Rheological analysis showed a 1.5 kPa increase in storage modulus, indicating enhanced mechanical integrity.
  • In vitro studies confirmed no cytotoxicity to human chondrocytes.
  • In vivo tracking demonstrated successful localized gelation and sustained delivery in an OA mouse model.

Conclusions:

  • The novel P407-peptide hydrogel presents a promising platform for intra-articular therapeutic delivery.
  • The formulation combines injectability, rapid thermoresponsive gelation, improved mechanical reinforcement, and controlled release capabilities.
  • This enhanced hydrogel is well-suited for regenerative medicine strategies and the treatment of osteoarthritis.