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Updated: Jun 18, 2026

$Microcrystallography of Protein Crystals and In Cellulo Diffraction$

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

Serial Chemical Crystallography for Autonomous Quantitative Phase Analysis in an Electron Microscope.

Taimin Yang^1,2, David Geoffrey Waterman^3,4, Zheting Chu¹

¹Department of Chemistry, Stockholm University, Svante Arrhenius väg 16C, Stockholm, SE-10691, Sweden.

|October 21, 2025

Summary

This summary is machine-generated.

We developed tilt serial electron diffraction (t-SerialED), a fast method for analyzing beam-sensitive nanomaterials. This technique enables high-throughput phase and structure determination for complex multi-phase systems.

Keywords:

SerialED autonomous data collection beam‐sensitive materials quantitative phase analysis serial crystallography

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Last Updated: Jun 18, 2026

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$Microcrystal Electron Diffraction of Small Molecules$

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

Area of Science:

Materials Science
Crystallography
Electron Microscopy

Background:

Characterizing beam-sensitive, nano-sized polycrystalline materials presents significant challenges.
Traditional serial crystallography workflows are time-consuming and not optimized for high-throughput analysis.

Purpose of the Study:

To introduce tilt serial electron diffraction (t-SerialED), a novel method for rapid, autonomous phase and structural analysis.
To enable high-throughput analysis of beam-sensitive, multi-phase materials, expanding beyond single-phase systems.

Main Methods:

t-SerialED utilizes a batch-by-batch data acquisition approach, significantly speeding up the process.
Combines robust 3D reciprocal space indexing with still-shot integration and merging techniques from serial crystallography.
Addresses key challenges in serial crystallography, including indexing and preferred orientation.

Main Results:

Demonstrated high-throughput analysis of diverse samples, including nanoporous frameworks and pharmaceutical compounds.
Enabled precise structure determination, visualizing guest molecules and non-covalent interactions like hydrogen bonding.
Successfully expanded serial chemical crystallography capabilities to complex multi-phase systems.

Conclusions:

t-SerialED offers a robust solution for routine quantitative phase analysis and structure determination of beam-sensitive nanomaterials.
This method serves as a valuable complementary technique to traditional crystallography, enhancing analytical throughput and scope.