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Updated: Jan 14, 2026

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
Published on: August 7, 2013
David R Linden1, Keith A Sharkey2
1Enteric Neuroscience Program, Mayo Clinic, Rochester, MN 55905, USA; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA.
The enteric nervous system (ENS), located in the gut, is a complex network with as many neurons as the spinal cord. This "second brain" can operate independently from the central nervous system.
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