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A Bayesian approach towards atomically-precise localization in fluorescence microscopy.

Yuqin Duan1, Qiushi Gu2, Hanfeng Wang2

  • 1Research Laboratory of Electronics, MIT, 50 Vassar Street, Cambridge, MA, 02139, USA. sophiayd@mit.edu.

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Researchers developed a new technique, discrete grid imaging (DIGIT), to overcome super-resolution microscopy limits. DIGIT achieves angstrom-level precision by utilizing atomic lattice structures for enhanced imaging of quantum and biological systems.

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Area of Science:

  • Physics
  • Optical Microscopy
  • Quantum Technology

Background:

  • Super-resolution microscopy surpasses the Abbe diffraction limit for imaging complex systems.
  • Single-molecule localization microscopy (SMLM) achieves nanometer precision but faces limitations.
  • Improving SMLM to angstrom-level resolution requires a significant, often impractical, increase in photon collection.

Purpose of the Study:

  • To overcome the fundamental barrier in SMLM resolution enhancement.
  • To develop a technique for achieving angstrom-level precision in optical imaging.
  • To enable direct optical resolution of atomic features in quantum and biological systems.

Main Methods:

  • Harnessing the periodic nature of atomic lattice structures.
  • Applying a discrete grid imaging technique (DIGIT).
  • Utilizing quantum emitter systems and large-scale quantum emitter arrays.

Main Results:

  • Observed an exponential collapse of localization uncertainty beyond the atomic lattice constant.
  • Demonstrated parallel positioning of emitters in large arrays via wide-field imaging.
  • Achieved angstrom-level precision, breaking previous resolution barriers.

Conclusions:

  • DIGIT overcomes the quadratic resource scaling limitations of SMLM.
  • The technique offers a competitive tool for unprecedented measurement precision.
  • DIGIT paves the way for direct optical resolution of atomic features in various systems.