Related Experiment Video
Updated: Jan 14, 2026
Author Spotlight: Developing Efficient Cryopreservation and Biobanking Technologies for Global Reef Restoration
Published on: June 7, 2024
Optimising Stallion Semen Cryopreservation: Preliminary Insights Into Pre-Centrifugation Extender Effects
Bruna Merci de Zutter1, Camila de Paula Freitas-Dell'Aqua1, José Antônio Dell'Aqua-Junior1
1Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
None:
This study evaluated the effects of cholesterol, pentoxifylline and casein, with or without skim milk, added to extenders during pre-centrifugation on equine sperm cryosurvival. Seven ejaculates from four stallions (n = 28) were divided into four groups: SM (skim milk), SMP (SM + pentoxifylline), SMCho (SM + cholesterol) and ChoCa (cholesterol + casein). After centrifugation and freezing, sperm kinematics and plasma membrane integrity were assessed immediately and 30 min post-thaw. SMCho and ChoCa showed superior results compared with SM and SMP. These findings indicate that cholesterol-based extenders improve post-thaw sperm quality when added before cryopreservation.
