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Oxygen Handling Practices for in Vitro Human Pluripotent Stem Cell Cardiomyocyte Differentiation: A Scoping
Lisse Frans1, Jeffrey Aalders2, Jolanda van Hengel3
1Department of Basic and Applied Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.
Oxygen handling during human pluripotent stem cell-derived cardiomyocyte (hPSC-CM) differentiation is frequently underreported. Standardization of oxygen conditions is crucial for improving reproducibility in cardiovascular research using these stem cell models.
Area of Science:
- Cardiovascular Research
- Stem Cell Biology
- Cellular Differentiation
Background:
- Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) are vital models for cardiovascular research.
- Optimizing hPSC-CM differentiation protocols is essential for mimicking native phenotypes.
- Variability in differentiation strategies necessitates standardization for reproducibility.
Purpose of the Study:
- To analyze oxygen handling practices in hPSC-CM differentiation over 15 years.
- To identify trends in reporting oxygen conditions during hPSC-CM differentiation.
- To highlight the underreporting of oxygen parameters in published research.
Main Methods:
- Systematic review of 1722 research articles on hPSC-CM differentiation.
- Analysis focused on oxygen handling (hypoxia/normoxia) during differentiation.
- Trend analysis of reporting accuracy over 15 years.
Main Results:
- Six main oxygen handling approaches were identified.
- Only 34% and 16% of articles explicitly reported oxygen conditions for hPSC culture and CM differentiation, respectively.
- Underreporting of oxygen conditions remained consistent over the study period.
Conclusions:
- Oxygen handling is a significantly underreported parameter in hPSC-CM differentiation research.
- Improved reporting and standardization of oxygen conditions are needed for reproducibility.
- Convergence towards common protocols suggests a move towards standardization.
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