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Updated: Jan 14, 2026

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
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Plasma-activated media inhibits epithelial-mesenchymal transition and ameliorates intestinal fibrosis through the

Yi You1, Yaping Shen1, Yan Yang1

  • 1Nanjing University of Chinese Medicine, Nanjing, China.

Plos One
|October 22, 2025
PubMed

Inflammatory bowel disease often complicates intestinal lumen stenosis, and intestinal fibrosis is the core pathological process leading to its development. Currently, there are no effective drug treatments available to prevent or improve intestinal fibrosis. Previous studies have shown that PAM (plasma-activated media) inhibits epithelial-mesenchymal transition (EMT) and improves skin fibrosis by regulating the PPARγ/TGF-β1 axis. However, it is unclear whether PAM can improve intestinal fibrosis. We used a gradient concentration of PAM to intervene in the dextran sulfate sodium (DSS)-induced mouse intestinal fibrosis model to evaluate its effects onalleviating fibrosis and explore the specific molecular mechanisms. In addition, we used PAM to intervene in the TGF-β1-induced rat intestinal crypt epithelial cell (IEC-6) EMT and fibrosis in an in vitro model to further explore the molecular mechanisms by which PAM improves intestinal fibrosis. We found that PAM can improve intestinal fibrosis by inhibiting epithelial-mesenchymal transition through the PPARγ/TGF-β1/SMAD signaling pathway.

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