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Updated: Jan 14, 2026
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A Model of Self-limited Acute Lung Injury by Unilateral Intra-bronchial Acid Instillation
Published on: August 30, 2019
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Specialized proresolving mediator-loaded extracellular vesicles mitigate pulmonary inflammation.
Manjula Karpurapu1, Jiasheng Yan2, Sangwoon Chung1
1Pulmonary, Critical Care and Sleep Medicine, Ohio State University Wexner Medical Center, Davis Heart and Lung Research Institute, Columbus, OH, United States.
American Journal of Respiratory Cell and Molecular Biology
|October 22, 2025
Summary
Researchers developed a new method to load extracellular vesicles (EVs) with specialized lipids called resolvins. These engineered EVs (Resolvin-EVs) effectively reduce inflammation and show promise for treating acute lung injury.
Area of Science:
- Biotechnology
- Synthetic Biology
- Nanomedicine
Background:
- Extracellular vesicles (EVs) are recognized for their therapeutic delivery capabilities.
- The potential of EVs for delivering bioactive lipid mediators is underexplored.
- Resolvins are crucial pro-resolving lipid mediators with anti-inflammatory properties.
Purpose of the Study:
- To develop a synthetic biology approach for engineering EVs loaded with resolvins.
- To investigate the therapeutic efficacy of these resolvin-loaded EVs (Resolvin-EVs) in inflammatory conditions.
Main Methods:
- Co-expression of resolvin biosynthetic enzymes (COX-2, 5-LOX, 15-LOX) in HEK293T cells using a multigene vector.
- Loading EVs with Resolvin D and E series mediators by culturing cells with DHA, EPA, and aspirin.
- Characterization of Resolvin-EVs and assessment of their effects on endothelial cells, macrophages, and in a mouse model of acute lung injury.
Main Results:
- Successfully generated Resolvin-EVs containing multiple resolvins and precursors.
- Resolvin-EVs attenuated neutrophil adhesion, preserved endothelial barrier integrity, and suppressed inflammatory cytokine release (IL6, TNFα) in macrophages.
- EV uptake was essential for Resolvin-EV therapeutic effects; enhanced efferocytosis was observed.
- In vivo administration of Resolvin-EVs reduced pulmonary inflammation in mice without toxicity.
Conclusions:
- A novel and scalable platform for producing resolvin-loaded EVs has been established.
- Resolvin-EVs demonstrate significant therapeutic potential for treating acute lung injury and chronic inflammatory diseases.
- This approach broadens the application of EVs as drug delivery vehicles for lipid mediators.
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