Related Experiment Video
Updated: Jan 14, 2026
Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection
Published on: September 18, 2020
Functional and phenotypic characterization of peripheral blood mononuclear cells from tuberculosis patients in
Pyae Sone Oo1, Jomkwan Ongarj1, Ratchanon Sophonmanee1
1Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
Introduction:
Tuberculosis (TB) remains a global health challenge, with active TB disease (ATB) and latent TB infection (LTBI) representing distinct immunological states. Understanding immune responses in these groups is critical for developing effective interventions. The complex nature of immune responses to Mycobacterium tuberculosis (M.tb) within and between different stages of TB, host evasion mechanisms of the bacterium, variable protection conferred by the BCG vaccine in adults, and lack of validated immune correlates of protection are among the key challenges to the successful control of TB.
Methods:
In the present study, we conducted functional and phenotypic characterization of peripheral blood mononuclear cells (PBMCs) from a cohort in Southern Thailand. We compared immune responses in individuals with ATB, LTBI and healthy controls (HC) using flow cytometry (ATB n = 9, LTBI n = 11, HC n = 10) and the mycobacterial growth inhibition assay (MGIA) (ATB n = 13, LTBI n = 15, HC n = 15).
Results:
MGIA revealed significantly enhanced control of BCG growth in the ATB group compared to LTBI and HC groups. Furthermore, NK cell frequency and TNF-α levels were significantly elevated in ATB compared to LTBI and HC groups, and CD4+ T cell TNF-α responses correlated with mycobacterial growth control.
Discussion:
The findings from this study demonstrate differential immune responses across TB stages in this cohort, identify potential cellular markers for TB diagnosis and monitoring, and may guide vaccine strategies and host-directed therapies.
More Related Videos
14:45Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
Published on: September 16, 2012
09:54Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
Published on: February 16, 2020
Related Concept Videos
01:26Pulmonary Tuberculosis IV
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
01:31Pulmonary Tuberculosis III
The first classification is based on the development of the disease, and it includes the following categories:
01:29Pulmonary Tuberculosis I
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
01:28Pulmonary Tuberculosis II
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
01:28Pulmonary Tuberculosis V
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...