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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Investigating the Molecular Epidemiology of Extended-Spectrum β-Lactamase-Producing Enterobacterales (ESBL-E) Among
Sima L Sharara1, Patricia J Simner2, Yehudit Bergman3
1Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Abstract:
At a United States hospital, sequencing of ICU rectal surveillance cultures indicated 5% ESBL-E colonization. Of confirmed ESBL isolates, 91% were Escherichia coli or Klebsiella pneumoniae; 6% carried non-bla CTX-M genes. Only 53% of third-generation cephalosporin-resistant Enterobacterales harbored ESBL genes, underscoring the limitations of phenotypic approaches as ESBL surrogates, particularly for non-E. coli/K. pneumoniae species.
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