Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunohistochemical staining using coupled immunofluorescence and immunocomplex technique.

M Brozman, D Chorváth, J Jakubovský

    Acta Histochemica
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Safety and outcomes of routine endovascular thrombectomy in large artery occlusion recorded in the SITS Register: An observational study.

    Journal of internal medicine·2021
    Same author

    Intravenous thrombolysis for patients with in-hospital stroke onset: propensity-matched analysis from the Safe Implementation of Treatments in Stroke-East registry.

    European journal of neurology·2017
    Same author

    The first imported human infestation with Furuncular myiasis in man in the Slovakia and current knowledge of myiasis.

    Bratislavske lekarske listy·2016
    Same author

    Cristobalite and Hematite Particles in Human Brain.

    Biological trace element research·2016
    Same author

    Ultrastructural changes of kidney in diabetic rats.

    Bratislavske lekarske listy·2016
    Same author

    Iron and its relation to glycoconjugates in human globus pallidus.

    Bratislavske lekarske listy·2014
    Same journal

    Involvement of the striped dolphin (Stenella coeruleoalba) melon in immune function: A histological and immunohistochemical study.

    Acta histochemica·2026
    Same journal

    YARS2 mediates PARPi resistance in pancreatic cancer by regulating the MYC-E2F axis.

    Acta histochemica·2026
    Same journal

    Developmental stage-specific localization of CK1α protein in the mouse ovary.

    Acta histochemica·2026
    Same journal

    Oleyl alcohol-enhanced embedding resin for histological analysis of non-decalcified hard tissue.

    Acta histochemica·2026
    Same journal

    Corrigendum to ''3D approach visualizing cellular networks in human lymph nodes'' [Acta Histochem. 120 (8) (2018) 720-727].

    Acta histochemica·2026
    Same journal

    YAP promotes wound healing in diabetic mice by improving endothelial cell function via the regulation of SEMA3B.

    Acta histochemica·2026
    See all related articles

    This study introduces a novel immunomicroscopic method combining fluorescent antibodies and enzyme immunocomplex techniques for simultaneous antigen and antibody localization. This advanced technique enhances immunofluorescence by enabling electron microscopy, improving diagnostic capabilities.

    Area of Science:

    • Immunohistochemistry
    • Molecular Biology
    • Microscopy

    Background:

    • Immunofluorescence is a common technique for visualizing antigens and antibodies in biological samples.
    • However, immunofluorescence has limitations, including potential signal fading and difficulty in achieving high resolution.
    • There is a need for improved methods that offer enhanced sensitivity and resolution for immunolocalization studies.

    Purpose of the Study:

    • To present a novel immunomicroscopic method.
    • To combine fluorescent antibody and enzyme immunocomplex techniques.
    • To enable simultaneous localization of antigens and antibodies in the same section.

    Main Methods:

    • The method involves sequential application of rabbit antiserum (IgG), FITC-conjugated antirabbit globulin, and a peroxidase-antiperoxidase complex.

    Related Experiment Videos

  • Detection is achieved using 3,3-diaminobenzidine and hydrogen peroxide.
  • This approach integrates immunofluorescence and immunoenzymatic detection.

    • Main Results:

    • The combined technique allows for simultaneous detection of both antigens and antibodies within the same tissue section.
    • It overcomes some limitations associated with traditional immunofluorescence techniques.
    • The method provides enhanced signal amplification and specificity.

    Conclusions:

    • This novel immunomicroscopic method offers a powerful tool for simultaneous antigen and antibody localization.
    • It improves upon existing immunofluorescence techniques by offering enhanced resolution and sensitivity.
    • The method has potential applications in diagnostics and research, including electron microscopic examination.