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Summary
Entamoeba invadens cells contain high zinc concentrations, particularly within chromatoid bodies in cysts. The exact role and chemical form of this zinc remain undetermined.
Area of Science:
- Microbiology
- Biochemistry
- Cell Biology
Background:
- Entamoeba invadens is a protozoan parasite.
- Chromatoid bodies in Entamoeba cysts are known to contain ribosome crystals.
Purpose of the Study:
- To quantify zinc concentration in Entamoeba invadens.
- To investigate the localization of zinc within the organism.
Main Methods:
- Atomic absorption spectroscopy
- Electron microprobe analysis
- Dithizone staining
Main Results:
- Trophozoites and cysts of Entamoeba invadens exhibit high zinc concentrations (approx. 10^-6 microgram/cell or 1% dry weight).
- In cysts, zinc is specifically localized within chromatoid bodies.
Conclusions:
- Entamoeba invadens accumulates significant amounts of zinc.
- Chromatoid bodies are a major zinc-containing structure in Entamoeba cysts.
- The chemical state and precise function of zinc in Entamoeba invadens require further investigation.