Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of Analytical Methods01:21

Development of Analytical Methods

1.7K
An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
1.7K
Data Validation01:15

Data Validation

572
Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
572
Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

2.5K
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
2.5K
Quantitative Analysis01:12

Quantitative Analysis

1.3K
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
1.3K
Therapeutic Drug Monitoring: Drug Analysis Methods01:26

Therapeutic Drug Monitoring: Drug Analysis Methods

176
Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
176
Standard Solutions01:14

Standard Solutions

2.3K
Standard solutions refer to solutions with a precisely known concentration or composition. A primary standard is a highly pure, high molar mass, stable substance that is entirely soluble in water, the most commonly used solvent in analytical chemistry. The primary standard solution can be used to standardize secondary standards, which are substances with known concentrations but are less pure and stable. Standard solutions are essential for achieving accurate and reliable results in analytical...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Charting the translational pathway: ISSCR best practices for the development of PSC-derived therapies.

Stem cell reports·2026
Same author

Interlaboratory assessment of candidate reference materials for lentiviral vector copy number and integration site measurements.

Molecular therapy. Methods & clinical development·2025
Same author

Scaling up pluripotent stem cell-based therapies - considerations, current challenges and emerging technologies: perspectives from the ISCT Emerging Regenerative Medicine Working Group.

Cytotherapy·2025
Same author

Analysis of the measurements used as potency tests for the 31 US FDA-approved cell therapy products.

Journal of translational medicine·2025
Same author

Advancing stem cell technologies for conservation of wildlife biodiversity.

Development (Cambridge, England)·2024
Same author

Bioprocessing considerations for generation of iPSCs intended for clinical application: perspectives from the ISCT Emerging Regenerative Medicine Technology working group.

Cytotherapy·2024
Same journal

Peptidomics in the Spotlight: Advanced Sample Treatment Techniques and Analytical Insights.

Advances in experimental medicine and biology·2026
Same journal

Methods for the Investigation of Protein-Ligands Interactions.

Advances in experimental medicine and biology·2026
Same journal

Sample Preparation Strategies for Microbial Cell Surface Proteomics: Integrating Shaving and Shotgun Approaches.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Sample Preparation for the Petroleum Industry: A Biocorrosion Case Study.

Advances in experimental medicine and biology·2026
Same journal

Proteomic and Functional Comparison of Extracellular Vesicles from Wild-Type and Lyn-Deficient Stromal Cells.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.

Advances in experimental medicine and biology·2026
See all related articles

Related Experiment Video

Updated: Jan 14, 2026

Applying Cheminformatics to Develop a Structure Searchable Database of Analytical Methods
05:34

Applying Cheminformatics to Develop a Structure Searchable Database of Analytical Methods

Published on: June 6, 2025

1.6K

Development of Standardized Methods and Analytical Tools.

Chad C MacArthur1, Uma Lakshmipathy2

  • 1Pharma Services, Thermo Fisher Scientific, San Diego, CA, USA.

Advances in Experimental Medicine and Biology
|October 24, 2025
PubMed
Summary
This summary is machine-generated.

Generating induced pluripotent stem cells (iPSCs) from adult cells is key for regenerative medicine. Optimizing methods, materials, and characterization ensures safe and effective iPSC therapies for personalized medicine.

Keywords:
Allogeneic therapiesAutologous therapiesCell banksClonal selectionCulture conditionsDrug discoveryEpisomal plasmidsGMP manufacturingInduced pluripotent stem cells (iPSCs)Regenerative medicineReprogramming methodsSendai virusSomatic reprogrammingXeno-free materialsmRNA reprogramming

More Related Videos

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
09:04

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

13.1K
PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.9K

Related Experiment Videos

Last Updated: Jan 14, 2026

Applying Cheminformatics to Develop a Structure Searchable Database of Analytical Methods
05:34

Applying Cheminformatics to Develop a Structure Searchable Database of Analytical Methods

Published on: June 6, 2025

1.6K
Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
09:04

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

13.1K
PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.9K

Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Induced pluripotent stem cells (iPSCs) offer significant potential for regenerative medicine and drug discovery.
  • Generating iPSCs involves reprogramming somatic cells, with diverse methods and conditions available.

Purpose of the Study:

  • To explore diverse methods and conditions for generating iPSCs from various adult cell sources.
  • To highlight the potential of iPSCs in autologous and allogeneic therapies.
  • To discuss key considerations for efficient and reproducible iPSC generation.

Main Methods:

  • Selection of appropriate reprogramming factors and systems.
  • Optimization of somatic cell sources and culture media conditions.
  • Implementation of robust methods for iPSC identification and characterization.

Main Results:

  • High-quality materials, defined processes, and analytical methods are crucial for iPSC safety, efficiency, and reproducibility.
  • Regulatory compliance, including xeno-free materials and guidelines, is essential.
  • Advances in automation are expected to improve process control and reduce variability.

Conclusions:

  • Combining qualified reagents, defined xeno-free workflows, and comprehensive characterization assays accelerates the clinical translation of iPSC technology.
  • This integration facilitates reliable and scalable regenerative therapies.
  • It significantly advances the field of personalized medicine.