Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prehospital whole blood after SWiFT and TOWAR: not superior, not settled.

Blood transfusion = Trasfusione del sangue·2026
Same author

Calling for Diversity: Improving Transfusion Safety Through High-Throughput Blood Group Microarray Genotyping.

Genomics, proteomics & bioinformatics·2026
Same author

Cord blood red cell concentrates for preterm neonate transfusion: Insights from the multicenter BORN trial.

Transfusion·2026
Same author

Association of blood group B and of rare variants affecting immune system with multisystem inflammatory syndrome in children in an Italian cohort.

Frontiers in immunology·2026
Same author

Competing gene regulatory networks drive naive and memory B cell differentiation.

Molecular systems biology·2026
Same author

Rheological and biochemical comparison of cord and adult blood red cells for transfusion applications.

Scientific reports·2026
Same journal

Recent Thymic Emigrant Levels in Inborn Errors of Immunity: Is Their Diagnostic Value Greater Than We Think?

Scandinavian journal of immunology·2026
Same journal

The EBNA-1 Conundrum: Does Epstein-Barr Virus Invoke Autoimmune Pathology in a Population Subset by Poorly Purine-Loading Its Major Latency-Maintaining Transcript?

Scandinavian journal of immunology·2026
Same journal

Unsupervised Flow Cytometry Reveals a Constant Shift Towards Activated CD4<sup>+</sup> T Cell Subsets in APECED.

Scandinavian journal of immunology·2026
Same journal

RETRACTION: Interleukin-13 Induces T Helper Type 2 Immune Responses in OVA-Immunized BALB/c Mice Bearing a T Cell Lymphoma.

Scandinavian journal of immunology·2026
Same journal

Development of a Detection Method for Soluble Urokinase-Type Plasminogen Activator Receptor and Its Application in Predicting Prognosis of Severe COVID-19.

Scandinavian journal of immunology·2026
Same journal

Crohn's Disease Enteritis: Pathophysiological Mechanisms and Therapeutic Approaches.

Scandinavian journal of immunology·2026
See all related articles

Related Experiment Video

Updated: Jun 19, 2026

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
08:17

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Published on: October 30, 2016

21.9K

Age-Matched Reference Values for Circulating Natural Killer T (NKT)-Like Cells.

Elena Trombetta1, Manuela Liguori1, Federico Simone Colombo1

  • 1Flow Cytometry Laboratory, Clinical Pathology, Fondazione IRCCS ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.

Scandinavian Journal of Immunology
|October 27, 2025
PubMed
Summary
This summary is machine-generated.

Natural Killer T-like (NKT-like) cells, crucial for innate immunity, show age-dependent changes. This study establishes reference values for NKT-like cell populations in adults and children, aiding in diagnosing immune-related conditions.

Keywords:
NKT‐like cellslymphocytesreference values

More Related Videos

A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
06:08

A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

Published on: August 9, 2017

25.0K
Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
06:55

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Published on: February 22, 2020

19.4K

Related Experiment Videos

Last Updated: Jun 19, 2026

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
08:17

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Published on: October 30, 2016

21.9K
A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
06:08

A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

Published on: August 9, 2017

25.0K
Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
06:55

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Published on: February 22, 2020

19.4K

Area of Science:

  • Immunology
  • Cell Biology
  • Clinical Diagnostics

Background:

  • Natural Killer T-like (NKT-like) cells are critical cytotoxic lymphocytes involved in innate immunity.
  • Imbalances in NKT-like cell distribution are linked to various pathologies, including cancers and autoimmune disorders.
  • Establishing age-matched reference values for NKT-like cell populations is essential for accurate clinical interpretation.

Purpose of the Study:

  • To define age-matched reference values for NKT-like cell counts and subpopulations in adults and children.
  • To investigate the age-related trends in NKT-like cell distribution, including CD4 and CD8 expression.
  • To provide a foundation for utilizing NKT-like cell data in clinical diagnostics.

Main Methods:

  • Flow cytometry with a lyse-no-wash protocol was used to analyze NKT-like cells in 2208 subjects.
  • Reference values were determined using data from 490 healthy adult and pediatric control groups across six age ranges.
  • NKT-like cell counts and expression of CD4/CD8 surface antigens were evaluated.

Main Results:

  • NKT-like cell numbers (percentage and absolute count) increase with age.
  • NKT-like/CD8+ cells increase with age, while NKT-like/CD4+ cells decrease.
  • Double-positive (CD4+CD8+) NKT-like cells decrease with age, whereas double-negative (CD4-CD8-) cells increase up to age 16 before decreasing in adults.

Conclusions:

  • This study provides the first robust, age-matched reference values for NKT-like cell subpopulations under CE-IVD conditions.
  • Defined reference ranges are crucial for identifying NKT-like cell count abnormalities associated with various diseases.
  • Incorporating NKT-like cell data into diagnostic reports can enhance the delineation of patient immune profiles.