Related Experiment Video
Updated: Jan 14, 2026

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
Published on: March 17, 2023
Donning Gloves Using the Sliding-Closed Technique Has a Larger Distance to Contamination at the Gown-Glove Interface
Hailee E Reist1, Michael B DiCosmo1, Luke C Seeker1
1Department of Orthopaedics and Rehabilitation, University of Vermont College of Medicine, Burlington, VT, USA.
The novel sliding-closed (SC) gloving technique significantly reduces bacterial contamination on surgical gloves compared to the traditional open technique. This optimized method enhances sterile barrier integrity, crucial for minimizing surgical site infections.
Area of Science:
- Surgical Infection Control
- Sterile Technique Optimization
- Medical Device Contamination
Background:
- The surgical gown-glove interface is a recognized source of bacterial contamination during procedures.
- Optimizing glove donning techniques, with sterile assistant aid, can minimize contamination risks.
- Current gloving methods may not fully prevent contamination at this critical interface.
Purpose of the Study:
- To compare bacterial contamination levels inside the glove cuff between a novel sliding-closed (SC) gloving technique and the traditional open gloving technique.
- To evaluate the effectiveness of the SC technique in maintaining a sterile barrier.
- To assess the impact of prior experience on the efficacy of the SC technique.
Main Methods:
- A comparative study was conducted using ultraviolet indicator lotion applied before donning sterile gloves and gowns.
- Surgeons utilized either the traditional open gloving technique or the novel SC technique.
- Contamination was quantified by measuring the distance from the glove cuff to the nearest contamination site, assessed by blinded inspectors.
Main Results:
- The SC technique demonstrated a significantly greater distance to contamination (150 mm) compared to the open technique (65 mm; P ≤ 0.001), irrespective of surgeon experience.
- Surgeons experienced with the SC technique showed a larger median distance to contamination (125 mm) than novices (90 mm; P = .002).
- The SC technique consistently provided a superior sterile barrier at the glove cuff.
Conclusions:
- The SC-assisted gloving technique significantly increases the distance from the glove cuff to contamination compared to the traditional open technique.
- Adoption of the SC technique is recommended to reduce infection risk in surgical settings.
- This technique serves as a valuable addition to existing infection control measures.
Related Concept Videos
PPE Use in Healthcare Settings I: Donning
PPE Use in Healthcare Settings II: Doffing
Handwashing III: During the Procedure and Post-Procedure Steps
Handwashing II: Pre-procedure and Initial Procedure Steps
The hand washing procedure itself includes the following steps. First, cover cuts, if any, on hands with a waterproof dressing. Cuts and abrasions can become contaminated with bacteria hindering the ability to clean the area thoroughly. In addition, repeated hand washing can worsen an injury. The nails must be short and clean, without nail...
Hand hygiene
Hand washing...
Personal Protective Equipment

