Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration
Amy M Kahler1, Mia C Mattioli2
1Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Atlanta, GA, USA. akahler@cdc.gov.
Methods in Molecular Biology (Clifton, N.J.)
|October 27, 2025
Abstract:
This procedure provides instructions for detection of Cryptosporidium from large-volume water samples. Water samples are collected by dead-end ultrafiltration in the field and processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to Cryptosporidium isolation or nucleic acid extraction methods for the detection of Cryptosporidium oocysts or Cryptosporidium deoxyribonucleic acid (DNA).
