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Optimized Genome Assembly Protocol for Cryptosporidium
1State Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
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Whole-genome sequence data have made significant contributions to studies of the biology and transmission of Cryptosporidium spp. Well-assembled genomes, free of sequence ambiguity and structural errors, are critical for comparative genomic analysis at the species and subtype levels. The optimized method for Cryptosporidium genome assembly described here comprises several individual protocols to generate chromosome- and contig-level genomes.
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