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Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

Subcellular Fractionation

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
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Updated: Jan 14, 2026

Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

Published on: November 3, 2009

Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation.

Michael J Arrowood^1,2

¹Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention (Retired), Atlanta, GA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 27, 2025

Summary

This summary is machine-generated.

This study details a method for purifying Cryptosporidium oocysts for laboratory research. Purified oocysts remain viable for over six months, enabling diverse research applications.

Keywords:

Centrifugation Cesium chloride Cryptosporidium Gradient Isolation Oocyst Purification Sheather’s solution Sucrose

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A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

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Last Updated: Jan 14, 2026

Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

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Published on: November 3, 2009

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A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Published on: July 9, 2012

Area of Science:

Parasitology
Infectious Diseases
Molecular Biology

Background:

Cryptosporidium oocysts are crucial for laboratory research.
Oocysts can be sourced from infected animals or humans.
Standard laboratory equipment can be used for purification.

Purpose of the Study:

To describe a reliable method for purifying Cryptosporidium oocysts.
To outline optimal storage conditions for purified oocysts.
To highlight the suitability of purified oocysts for various research applications.

Main Methods:

Oocyst purification using table-top centrifuges and microcentrifuges.
Storage in antibiotic-supplemented buffers or potassium dichromate.
Assessment of oocyst viability and infectivity over time.

Main Results:

Purified oocysts remain viable for over six months.
Oocyst viability declines significantly after one year.
Serial passage in hosts is recommended for long-term isolate maintenance.

Conclusions:

A standardized method for oocyst purification is presented.
Purified oocysts are suitable for diverse research, including animal studies, cell cultures, and molecular analyses.
Proper storage and maintenance are essential for preserving oocyst viability and infectivity.