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Updated: Jan 14, 2026
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High-density Electroencephalographic Acquisition in a Rodent Model Using Low-cost and Open-source Resources
Published on: November 26, 2016
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A Cost-Effective and Minimally Invasive Protocol for Chronic Multi-Site Electroencephalography Recording in Freely
Kazi Rafsan Radeen1, Khadijah Shanazz2, Caili Hao3
1Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University; National Institute of Biotechnology.
Journal of Visualized Experiments : Jove
|October 27, 2025
Summary
We developed a cost-effective, minimally invasive method for chronic, multi-site cortical electroencephalography (EEG) recordings in freely moving mice, enabling long-term brain activity monitoring for neurological disorder research.
Area of Science:
- Neuroscience
- Biomedical Engineering
Background:
- Electroencephalography (EEG) is crucial for brain activity monitoring in clinical and research settings.
- Existing rodent EEG systems face limitations in cost, invasiveness, and technical complexity for long-term studies.
Purpose of the Study:
- To present a reliable, accessible, and economical protocol for chronic, multi-site cortical EEG acquisition in freely moving mice.
- To enable long-term brain network dynamics investigation in rodent models.
Main Methods:
- Fabrication of platinum-iridium wire screw electrodes connected to a USB interface.
- Epidural anchoring using bone screws, secured with adhesive and dental cement.
- Chronic implantation in C57BL/6J mice, EEG sampling at 1,000 Hz, filtered 1-500 Hz.
Main Results:
- Stable signal capture over several months with an 85% post-operative survival rate.
- Successful induction of seizure-like activity with pentylenetetrazol, showing a significant increase in interictal discharges (2-5 to 20-51 events).
- Increased signal amplitude correlated with seizure activity.
Conclusions:
- The developed protocol offers a stable, adaptable, and economically viable platform for rodent EEG research.
- This method supports integration with behavioral tracking and various experimental manipulations.
- Facilitates advanced research in epilepsy and other neurological disorders using rodent models.
