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Updated: Jan 14, 2026

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands
Published on: January 31, 2025
Distinct methane release patterns associated with two wetlands emergent plants: microbial community in root biofilm
Wenjie Yuan1, Songhe Zhang1, Shaozhuang Guo1
1Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, China; College of Environment, Hohai University, Nanjing, 210098, China.
Abstract:
Plant-mediated methane (CH4) transport is a significant emission pathway in wetlands, facilitated by the aerenchyma of emergent plants. This study demonstrates contrasting effects of Sagittaria Sagittifolia (SAG) and Ludwigia leptocarpa (LUD) on system CH4 fluxes under different ambient air temperature. Above 20 °C, SAG exhibited negligible influence on CH4 fluxes but LUD significantly enhanced CH4 fluxes compared to bare sediment, although LUD has a lower aerenchyma proportion than SAG. When temperature was below 20 °C, two plants did not promote CH4 fluxes. The CH4 concentrations peaked in sediment with the highest number of plant root tips in SAG and LUD treatments. Root biofilms exhibited more complex microbial interactions and higher deterministic processes than rhizosphere sediments. Microenvironments shaped by root influenced the distribution of methanogens and methanotrophs in the sediment system, and the methanogenic pathways were mainly hydrogenotrophic in root biofilms, while acetotrophic and hydrogenotrophic methanogenic pathways shared the equivalent existence in rhizosphere sediments. Temperature affected the adundances of the mcrA and pmoA genes, explained the differences in sediment CH4 concentrations and CH4 fluxes, while gene abundance and aerenchyma proportions had a lesser effect than temperature.
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