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Overview of Metabolism01:40

Overview of Metabolism

Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...

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Comprehensive Metabolomic and Transcriptomic Analyses Reveal SlSnRK2.6/SlCHPP-SlbHLH95-SlPDS Module Regulating Tomato

Chunping Jia1, Haitao Yang1, Tao Yang1

  • 1Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Institute of Fruits and Vegetables, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.

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|October 28, 2025
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Summary

A tomato mutant with higher lycopene content reveals a new regulatory module for carotenoid biosynthesis. This SlSnRK2.6/SlCHPP-SlbHLH95-SlPDS pathway enhances fruit quality.

Keywords:
carotene biosynthesismultiomics combined analysisregulatory modulestomato fruit qualitytranscription factors

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Area of Science:

  • Plant Biology
  • Biochemistry
  • Genetics

Background:

  • Carotenoids are vital for fruit appearance and nutritional value.
  • Tomato fruit quality is significantly influenced by carotenoid accumulation.

Purpose of the Study:

  • To elucidate the molecular mechanisms regulating carotenoid biosynthesis in tomato.
  • To identify key regulators involved in lycopene accumulation.

Main Methods:

  • Comparative analysis of a tomato mutant (xh49♂-cr-1) with wild type.
  • Integrated metabolome and transcriptome analyses.
  • Molecular validation of gene and protein interactions.

Main Results:

  • The mutant exhibited significantly higher lycopene content due to upregulated phytoene and lycopene during ripening.
  • Transcription factor SlbHLH95 was identified as a key positive regulator of carotenoid biosynthesis.
  • A regulatory module involving SlSnRK2.6, SlCHPP, SlbHLH95, and SlPDS was characterized, forming a feedback and feed-forward loop.

Conclusions:

  • The SlSnRK2.6/SlCHPP-SlbHLH95-SlPDS module is a novel regulator of carotenoid biosynthesis in tomato.
  • Understanding this pathway provides a basis for improving tomato fruit quality through genetic strategies.