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Delignification of the Paddy Husk Using Binary and Ternary Deep Eutectic Solvents
Dilsad Dolunay Eslek Koyuncu1, Mujgan Okur1
1Faculty of Engineering, Chemical Engineering Department, Gazi University, Ankara, Turkey.
Biotechnology and Applied Biochemistry
|October 28, 2025
Summary
Deep eutectic solvents (DESs) effectively pretreat paddy husk for enhanced biomass conversion. Ternary DES systems, particularly ethylene glycol/citric acid/1-butanol, show superior delignification and enzymatic hydrolysis efficiency.
Area of Science:
- Biomass Pretreatment
- Green Chemistry
- Biorefining
Background:
- Lignocellulosic biomass pretreatment is essential for efficient hydrolysis and monomer conversion.
- Deep eutectic solvents (DESs) offer an economical, non-toxic, and biodegradable alternative for biomass pretreatment.
Purpose of the Study:
- Investigate the pretreatment and delignification of paddy husk using binary and ternary DES.
- Evaluate the impact of DES composition and reaction time on lignin removal and enzymatic hydrolysis.
Main Methods:
- Preparation of binary and ternary DES systems using citric acid monohydrate (CA), ethylene glycol (EG), choline chloride (ChCl), and 1-butanol (1but).
- Pretreatment of paddy husk with DES at 110°C for 2 and 4 hours.
- Analysis of cellulose, hemicellulose, and lignin content (NREL/TP-510-42618), structural changes (FT-IR, XRD), and morphology (FE-SEM).
- Assessment of enzymatic hydrolysis efficiency.
Main Results:
- The EG/CA/1but ternary DES system achieved the highest lignin removal (60.58%) at 2 hours.
- Ternary DES systems generally exhibited higher lignin removal efficiencies than binary systems.
- Lignin removal ranged from 47.72% to 60.58% across the tested DES systems.
- Enzymatic hydrolysis efficiency correlated positively with lignin removal percentages.
Conclusions:
- EG/CA/1but DES systems are effective for delignification and enhancing enzymatic hydrolysis of paddy husk.
- DES-based pretreatment offers a promising route for sustainable biomass valorization.
