Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular dosimetry of DNA adducts in mice exposed to ethylene oxide.

Toxicological sciences : an official journal of the Society of Toxicology·2026
Same author

Molecular dosimetry of hemoglobin adducts in mice exposed to ethylene oxide.

Archives of toxicology·2026
Same author

High-Throughput Suspect Screening Reveals Widespread Pesticide Exposure in Early Childhood.

Environment & health (Washington, D.C.)·2026
Same author

Activity-guided substructure prioritization accelerates discovery of gut microbiota-derived immune-regulating metabolites.

Nature communications·2026
Same author

Sensitive Quantification of the Ethylene Oxide Hemoglobin Adduct <i>N</i>-(2-Hydroxyethyl)-l-valine from Minimal Blood Volumes.

Chemical research in toxicology·2026
Same author

Current Status of Per- and Poly-Fluoroalkyl Substances (PFAS) Exposure on Lung Cell Biology and Pulmonary Outcomes along Human Health Risk Assessment Steps.

Current allergy and asthma reports·2026

Related Experiment Video

Updated: Jun 24, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.3K

Development of LC-MS/MS Database Based on 250 Potentially Highly Neuroactive Compounds and Their Metabolites.

Taylor Teitelbaum1, Haoduo Zhao2, Lauren E Koval2

  • 1Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Metabolites
|October 28, 2025
PubMed
Summary

A new database identifies environmental chemicals and their metabolites, aiding research into neurodevelopmental disorders. This tool enhances the detection of potentially harmful compounds in biological samples.

Keywords:
databaseexposomicsmass spectrometrymetabolitesneuroactive

More Related Videos

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.6K
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

9.1K

Related Experiment Videos

Last Updated: Jun 24, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.3K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.6K
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

9.1K

Area of Science:

  • Environmental Health
  • Toxicology
  • Neuroscience

Background:

  • Environmental chemicals are linked to neurodevelopmental disorders, but testing is limited.
  • The Environmental NeuRoactIve Chemicals (ENRICH) list prioritizes 250 chemicals for neuroactivity and exposure assessment.
  • Detecting these chemicals and their metabolites in humans is challenging due to data gaps.

Purpose of the Study:

  • To create a comprehensive LC-MS/MS database of environmental chemicals and their metabolites.
  • To support the identification and quantification of neuroactive environmental compounds in biological samples.

Main Methods:

  • Developed an LC-MS/MS database using the ENRICH list targets.
  • Utilized S9 human liver fractions for in vitro metabolism studies.
  • Identified novel and known phase I metabolites of prioritized chemicals.

Main Results:

  • The database includes 274 compounds: 94 parent chemicals and 182 metabolites.
  • Discovered 55 novel metabolites.
  • Achieved high confidence annotations (Levels 1-3) for 87% of the database, facilitating accurate identification.

Conclusions:

  • The database enables future studies on the impact of environmental chemicals and metabolites on brain health.
  • Facilitates a deeper understanding of the origins of neurodevelopmental disorders.
  • Supports exposomic research for improved environmental health assessments.