Pennisetum glaucum (L.) Oral Supplementation Mitigates Multi-Organic Dysfunction Associated with Carcinogenesis in
Paula A Oliveira1,2, Latifa Hajri3, Armando V Pinto Moreno4
1Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal.
Abstract:
Cancers induced by human papillomavirus are often associated with systemic inflammation and cachexia. This study aimed to determine the interference of Pennisetum glaucum oral supplementation over multi-organic dysfunction in HPV16-transgenic mice. The experimental groups included (1) wildtype (WT) mice with standard diet, (2) WT mice with 36% Pennisetum, (3) transgenic mice with standard diet, (4) transgenic mice with 29% Pennisetum, and (5) transgenic mice with 36% Pennisetum. During the 4-week experimental protocol, body weight, food and water intake, and humane endpoints were recorded. At sacrifice, blood and tissue samples were collected for analysis. Oral supplementation with millet was shown to be safe and well tolerated by both WT and transgenic mice, with no adverse effects on behavior, food or water intake, or general animal welfare. In HPV16-transgenic animals, millet supplementation was associated with an improved health status, reduced serum glucose levels, enhanced antioxidant responses, and a notable reduction in the severity of HPV-induced skin and organ lesions. Overall, Pennisetum glaucum was safe under these experimental conditions and is a promising functional food for patients suffering from systemic paraneoplastic syndromes. Longer exposure periods and doses should be evaluated experimentally before proceeding to clinical trials of Pennisetum-containing diets.
Insights
Oral supplementation with Pennisetum glaucum (millet) is safe and may improve health in mice with human papillomavirus-induced cancers. Millet reduced inflammation and lesions, showing promise for paraneoplastic syndromes.
Area of Science:
- Biomedical Science
- Oncology
- Nutritional Science
Background:
- Human papillomavirus (HPV)-induced cancers often involve systemic inflammation and cachexia, leading to multi-organ dysfunction.
- Investigating natural interventions is crucial for managing these complex oncological conditions.
Purpose of the Study:
- To evaluate the effects of Pennisetum glaucum (millet) oral supplementation on multi-organ dysfunction in HPV16-transgenic mice.
- To assess the safety and tolerability of millet supplementation in both wildtype and transgenic mouse models.
Main Methods:
- Four-week oral supplementation study involving wildtype and HPV16-transgenic mice fed standard or millet-supplemented diets.
- Monitoring of body weight, food/water intake, and animal welfare.
- Analysis of blood and tissue samples post-sacrifice to assess health status, glucose levels, antioxidant responses, and lesion severity.
Main Results:
- Pennisetum glaucum supplementation was safe and well-tolerated, with no adverse effects on animal behavior or welfare.
- In HPV16-transgenic mice, millet supplementation improved health status, reduced serum glucose, enhanced antioxidant responses, and decreased HPV-induced skin and organ lesion severity.
- The study demonstrated a beneficial impact of millet on mitigating multi-organ dysfunction associated with HPV-induced cancers.
Conclusions:
- Pennisetum glaucum is a safe functional food candidate for managing systemic paraneoplastic syndromes associated with HPV-induced cancers.
- Further research involving longer exposure periods and varied doses is warranted before clinical trials.
- Millet shows potential as a dietary intervention to improve health outcomes in cancer patients experiencing cachexia and inflammation.
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