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Updated: Jan 13, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Time-resolved photon counting Fourier-transform micro-spectroscopy enables simultaneous Raman and fluorescence

Lindong Shang^1,2,3, Xiaodong Bao^1,2,3, Hao Peng^1,2,3

¹Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, China.

Light, Science & Applications

|October 29, 2025

Summary

We developed a new spectroscopy platform to overcome limitations in time-gated Raman spectroscopy. This instrument effectively separates Raman and fluorescence signals with high spectral resolution and wide spectral range.

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Last Updated: Jan 13, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

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Published on: December 30, 2025

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

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Published on: February 9, 2012

Fluorescence Lifetime Macro Imager for Biomedical Applications

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Published on: April 7, 2023

Area of Science:

Spectroscopy
Photonics
Analytical Chemistry

Background:

Time-gated Raman spectroscopy has limitations including narrow spectral range and low spectral resolution.
Simultaneous acquisition of Raman and fluorescence lifetime images is challenging.

Purpose of the Study:

To develop a Fourier-transform photon counting spectroscopy platform.
To overcome limitations of existing time-gated Raman spectroscopy methods.
To simultaneously acquire Raman and fluorescence lifetime images.

Main Methods:

A Mach-Zehnder interferometer with a high-accuracy linear motor stage was utilized.
Photon-counting avalanche diodes and time-tagged acquisition were employed.
Photons were sorted based on coarse arrival time (50 ns steps) and fine arrival time (80 ps resolution).

Main Results:

Achieved a time resolution of 547 ps.
Obtained a wide spectral range of -1000 to 10,000 cm^-1 Raman shift.
Reached a high spectral resolution of 0.05 cm^-1.
Successfully separated Raman signals (during excitation pulse) from fluorescence signals (after excitation pulse).

Conclusions:

The developed Fourier-transform photon counting spectroscopy platform effectively addresses limitations in time-gated Raman spectroscopy.
The instrument demonstrates capability for simultaneous Raman and fluorescence signal acquisition and separation.
Validated using fluorescently coated silicon wafers and microspheres.