GRE: A Framework for Significant SNP Identification Associated with Wheat Yield Leveraging GWAS-Random Forest Joint...

Background: Facing global wheat production pressures such as environmental degradation and reduced cultivated land, breeding innovation is urgent to boost yields. Genomic selection (GS) is a useful wheat breeding technology to make the breeding process more efficient, increasing the genetic gain per unit time and cost. Precise genomic estimated breeding value (GEBV) via genome-wide markers is usually hampered by high-dimensional genomic data. Methods: To address this, we propose GRE, a framework combining genome-wide association study (GWAS)'s biological significance and random forest (RF)'s prediction efficiency for an explainable machine learning GS model. First, GRE identifies significant SNPs affecting wheat yield traits by comparison of the constructed 24 SNP subsets (intersection/union) selected by leveraging GWAS and RF, to analyze the marker scale's impact. Furthermore, GRE compares six GS algorithms (GBLUP and five machine learning models), evaluating performance via prediction accuracy (Pearson correlation coefficient, PCC) and error. Additionally, GRE leverages Shapley additive explanations (SHAP) explainable techniques to overcome traditional GS models' "black box" limitation, enabling cross-scale quantitative analysis and revealing how significant SNPs affect yield traits. Results: Results show that XGBoost and ElasticNet perform best in the union (383 SNPs) of GWAS and RF's TOP 200 SNPs, with high accuracy (PCC > 0.864) and stability (standard deviation, SD < 0.005), and the significant SNPs identified by XGBoost are precisely explained by their main and interaction effects on wheat yield by SHAP. Conclusions: This study provides tool support for intelligent breeding chip design, important trait gene mining, and GS technology field transformation, aiding global agricultural sustainable productivity.