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Updated: Jan 13, 2026
08:14
Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
Published on: September 29, 2015
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Effects of Platelet-Rich Fibrin on In Vitro Periodontal Ligament Cell Functions
Pablo Cores Ziskoven1, Andressa Vilas Boas Nogueira1, Jean-Claude Imber2
1Department of Periodontology and Operative Dentistry, University Medical Center, Johannes Gutenberg University, 55131 Mainz, Germany.
Biomedicines
|October 29, 2025
Summary
Platelet-rich fibrin (PRF) enhances periodontal wound healing by stimulating key molecules in periodontal ligament cells. Its beneficial effects on cell migration and gene expression persist even in inflammatory conditions, offering a promising regenerative therapy.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Periodontology
Background:
- Periodontitis causes tissue destruction and tooth loss.
- Regenerative therapies aim to restore lost periodontal tissues.
- Platelet-rich fibrin (PRF) is a potential regenerative agent, but its efficacy in inflammatory conditions requires clarification.
Purpose of the Study:
- To investigate the effects of PRF on periodontal ligament (PDL) cells.
- To assess PRF's impact on genes vital for periodontal wound healing and tissue remodeling.
- To determine PRF's efficacy under inflammatory conditions induced by TNF-α.
Main Methods:
- Real-time PCR and ELISA were used to analyze gene expression (VEGF, BMP2, COX2, TNF-α, SPP1).
- PDL cells were treated with varying concentrations of PRF, with and without TNF-α.
- Cell viability, proliferation, and migration assays were conducted.
Main Results:
- PRF significantly increased the expression of VEGF, BMP2, COX2, TNF-α, and SPP1 in a dose-dependent manner.
- These PRF-induced effects were not modulated by the presence of TNF-α, indicating efficacy in inflammation.
- PRF significantly enhanced cell migration by 35% without affecting viability or proliferation.
Conclusions:
- PRF promotes periodontal wound healing by upregulating crucial genes like VEGF, BMP2, and SPP1.
- PRF's pro-regenerative effects are maintained in an inflammatory environment.
- PRF demonstrates potential as a therapeutic agent for periodontitis treatment.
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