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Comprehensive Transcriptomic Analysis of the Molecular Mechanisms Conferring Resistance to Rice Blast in the Elite
Shuijin Zhang1, Yinyin Mao2, Yonghe Hong1
1Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350002, China.
International Journal of Molecular Sciences
|October 29, 2025
Summary
Rice blast resistance in elite hybrid rice line Fuhui2165 (FH2165) was studied. FH2165 and Huahangsimiao (HHSM) showed stronger resistance, linked to energy metabolism and RNA processing pathways.
Area of Science:
- Plant Pathology
- Molecular Biology
- Genetics
Background:
- Rice blast, caused by Magnaporthe oryzae, poses a significant threat to global food security.
- Developing rice varieties with enhanced blast resistance is crucial for sustainable agriculture.
- The resistance mechanisms of the elite restorer line Fuhui2165 (FH2165) are not well understood.
Purpose of the Study:
- To investigate the rice blast resistance of FH2165 and its parental lines.
- To elucidate the molecular mechanisms underlying blast resistance in FH2165 using transcriptome analysis.
- To identify potential targets for breeding high-yield, disease-resistant hybrid rice.
Main Methods:
- Phenotypic analysis of rice blast resistance in FH2165, Huahangsimiao (HHSM), Minghui86 (MH86), and Shuhui527 (SH527).
- Transcriptome sequencing analysis to identify differentially expressed genes (DEGs).
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses.
Main Results:
- FH2165 and HHSM exhibited superior blast resistance compared to MH86 and SH527.
- Transcriptome analysis revealed significant differences in gene expression, with DEGs enriched in chloroplast, plastid, and thylakoid functions.
- KEGG analysis indicated enrichment in carbon metabolism, amino acid biosynthesis, and photosynthesis pathways, suggesting energy reprogramming and secondary metabolite synthesis are key defense strategies. Specific co-expression in FH2165 and HHSM pointed to RNA processing, GTP binding, and purine metabolism.
Conclusions:
- The study identified key metabolic and signaling pathways, including energy metabolism and RNA processing, contributing to FH2165's blast resistance.
- Findings provide insights into the molecular basis of rice blast resistance.
- This research offers potential targets for developing improved hybrid rice varieties with enhanced disease resistance and yield.
