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Updated: Jan 13, 2026
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Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
Published on: May 9, 2020
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UCHL1 Promotes Gastric Cancer Progression by Regulating CIP2A Degradation
Ga-Ye Lee1, In-Ho Jeong1, Byung Sik Kim2
1Department of Biochemistry & Molecular Biology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea.
Pharmaceuticals (Basel, Switzerland)
|October 29, 2025
Summary
Uch-L1 (ubiquitin C-terminal hydrolase L1) deubiquitinating enzyme promotes gastric cancer progression by upregulating CIP2A and c-Myc signaling. Targeting Uch-L1 may offer a new therapeutic strategy for gastric cancer patients.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Gastric cancer is a leading cause of cancer mortality globally.
- Protein ubiquitination/deubiquitination are crucial post-translational modifications in tumorigenesis.
- The role of deubiquitinating enzyme UCHL1 in gastric cancer was previously unclear.
Purpose of the Study:
- To investigate the role and mechanism of UCHL1 in gastric cancer progression.
- To determine the prognostic significance of UCHL1 in gastric cancer patients.
- To explore UCHL1 as a potential therapeutic target.
Main Methods:
- Kaplan-Meier analysis and analysis of gastric cancer patient tissues.
- In vitro assays including cell viability, colony formation, migration, and invasion.
- Immunoprecipitation and Western blotting to analyze protein interactions and expression.
Main Results:
- UCHL1 expression is significantly upregulated in gastric cancer tissues and associated with poor prognosis.
- Reduced UCHL1 expression inhibits gastric cancer cell proliferation, migration, and invasion.
- UCHL1 deubiquitinates and stabilizes CIP2A, an oncogenic regulator of c-Myc, promoting cell cycle progression.
Conclusions:
- UCHL1 acts as an oncogenic factor promoting gastric cancer progression.
- UCHL1 influences gastric cancer via the CIP2A/c-Myc pathway, affecting cell cycle regulators like cyclin D1.
- UCHL1 represents a promising therapeutic target for gastric cancer treatment.
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