Phase-Specific Alterations in Gut Microbiota and Their Associations with Energy Intake and Nutritional Clustering in...

Background/Objectives: This study investigated how phase-specific dietary strategies and weight regulation influence gut microbiota composition and diversity in competitive weightlifters. Particular emphasis was placed on integrating energy intake, macronutrient clustering, and weight fluctuations across distinct training phases. Methods: Thirteen competitive weightlifters were recruited, with 10-12 contributing complete data per phase. Fecal and dietary samples were collected during the preparation, competition, and transition phases. Gut microbiota was profiled via 16S rRNA gene sequencing, and alpha/beta diversity was analyzed using QIIME2. K-means clustering based on caloric/macronutrient intake identified dietary patterns. Taxonomic differences were assessed using DESeq2, and microbial structures were compared across training phases, weight classes, and weight-change categories. Results: Overall phylum- and genus-level profiles and diversity indices remained stable across training phases, indicating community-level resilience. However, specific genera varied with dietary and physiological factors. Enterococcus was higher during the preparation phase, whereas Lactobacillus was enriched during the competition and transition phases as well as in the high-calorie cluster. Lightweight and heavyweight athletes also showed distinct microbial structures, and pre- and post-competition weight changes were associated with shifts in selected taxa. Notably, the low-calorie group exhibited higher Shannon diversity than the high-calorie group (p = 0.0058), with Lactobacillus dominance contributing to reduced evenness in high-energy diets. Conclusions: Despite overall microbial stability, dietary energy availability and body-weight regulation modulated specific taxa relevant to performance and recovery. By integrating dietary clustering, weight-class comparison, and pre- and post-competition weight changes, this study provides novel insight into the microbiota of resistance-trained athletes, a population underrepresented in previous research. Despite the modest sample size and single-season scope, this study offers new evidence linking dietary strategies, weight regulation, and gut microbiota in weightlifters, and highlights the need for validation in broader cohorts.