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Updated: Jan 13, 2026
Remote Laboratory Management: Respiratory Virus Diagnostics
Published on: April 6, 2019
Elimination of Airborne Microorganisms Using Compressive Heating Air Sterilization Technology (CHAST): Laboratory and
Pritha Sharma1, Supriya Mahajan2, Gene D Morse3
1Department of Medicine, Yale New Haven Health, New Haven, CT 06610, USA.
Background:
Airborne transmission of bacteria, viruses, and fungal spores poses a major threat in enclosed settings, particularly nursing homes where residents are highly vulnerable. Compressive Heating Air Sterilization Technology (CHAST) applies compressive heating to inactivate microorganisms without reliance on filtration or chemicals.
Methods:
CHAST efficacy was evaluated in laboratory and deployed for a feasibility and performance validation study of air sterilization in a nursing home environment. Laboratory studies tested prototypes (300-5000 CFM; 220-247 °C) against aerosolized surrogates including Bacillus globigii (Bg), B. stearothermophilus (Bst), B. thuringiensis (Bt), Escherichia coli, and MS2 bacteriophage. Viral inactivation thresholds were further assessed by exposing MS2 to progressively lower treatment temperatures (64.5-143 °C). Feasibility and performance validation evaluation involved continuous operation of two CHAST units in a nursing home, with pre- and post-treatment air samples analyzed for bacterial and fungal burden.
Results:
Laboratory testing demonstrated consistent microbial inactivation, with most prototypes achieving > 6-log (99.9999%) reductions across bacterial spores, vegetative bacteria, and viruses. A 5000 CFM prototype achieved > 7-log (99.99999%) elimination of B. globigii. MS2 was completely inactivated at 240 °C, with modeling suggesting a threshold for total viral elimination near 170 °C. In the feasibility study, baseline sampling revealed bacterial (35 CFU/m3) and fungal (17 CFU/m3) contamination, dominated by Bacillus, Staphylococcus, Cladosporium, and Penicillium. After 72 h of CHAST operation, discharge air contained no detectable viable organisms, and fungal spore counts showed a 93% reduction relative to baseline return air. Units maintained stable operation (464 °F ± 2 °F; 329-335 CFM) throughout deployment.
Conclusion:
CHAST reproducibly and scalably inactivated airborne bacteria, viruses, and fungi under laboratory and feasibility field studies, supporting its potential as a chemical-free strategy to improve infection control and indoor air quality in healthcare facilities.
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