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Updated: May 5, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
Structured RDF dataset for genomic feature extraction and detection of biotechnological microorganisms of the
Reynold Osuna-González1, Guillermo De Ita Luna1, Rosa María Valdovinos-Rosas2
1Facultad de Ciencias de la Computación, Benemérita Universidad Autónoma de Puebla, Avenida San Claudio s/n, Ciudad Universitaria, La Hacienda C.P. 72592, Puebla, Puebla, Mexico.
Abstract:
The current dataset has been built from 200 genomes of Burkholderia genus microorganisms in GenBank Flat File (GBFF) format, with information obtained from the National Center for Biotechnology Information (NCBI). Python scripts were developed to automate the extraction of genomic features such as coding sequences (CDS) and different types of RNA, extracting the descriptive information of each one. The extracted features were transformed into RDF (Resource Description Framework) format as initial knowledge graphs with Turtle syntax, and later, they were merged into a unified knowledge graph (KG) to facilitate their access via queries written in the SPARQL system. This dataset is publicly available to researchers through the Mendeley repository, allowing them to perform complex searches, looking for composite features coming from the dataset's source organisms. Choosing the RDF format for the knowledge graph also promotes interoperability with other biological datasets that use the same structure and are widely accessible through SPARQL Endpoints.
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