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Related Experiment Video

Updated: Jan 13, 2026

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
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Photo-Induced Ultra-Fast Duplex Invasion Targeting Long-Range Double-Stranded DNA Using Artificial Nucleotide.

Zumila Hailili1, Yasuha Watanabe1, Siddhant Sethi1

  • 1Bioscience, Biotechnology and Biomedical Engineering Research Area, Japan Advanced Institute of Science and Technology, Asahidai 1-1, Nomi, Ishikawa, 923-1292, Japan.

Chembiochem : a European Journal of Chemical Biology
|October 29, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a rapid photo-induced duplex invasion (pDI) method for genomic DNA manipulation. This new technique uses artificial nucleotides for fast and efficient sequence-selective DNA targeting.

Keywords:
CNVKDNAduplex invasionnucleic acidsphoto‐crosslinker

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Genomic DNA is crucial for biological processes, and its abnormal expression can lead to genetic diseases.
  • Sequence-selective DNA recognition is essential for genetic manipulation.
  • Photo-induced double-duplex invasion (pDDI) using artificial nucleotides (CNVK, CU) was previously developed.

Purpose of the Study:

  • To investigate the invasion mechanism of pDDI.
  • To develop a faster and more efficient method for DNA manipulation.

Main Methods:

  • Investigated the invasion mechanism of pDDI.
  • Developed a novel photo-induced duplex invasion (pDI) approach using 3-cyanovinylcarbazole nucleoside (CNVK).

Main Results:

  • Discovered invasion independence of pDDI probes.
  • The new pDI method achieves rapid DNA invasion using only CNVK.
  • High invasion efficiency was maintained with the pDI method.

Conclusions:

  • The rapid pDI approach offers a powerful new tool for site-specific genomic DNA manipulation.
  • This method enhances efficiency and speed in genetic engineering applications.