Photo-Induced Ultra-Fast Duplex Invasion Targeting Long-Range Double-Stranded DNA Using Artificial Nucleotide

Genomic DNA stores genetic information and regulates functions of various biological processes. Genetic diseases can be caused by abnormal gene expression. Genetic manipulation requires sequence-selective recognition of double-stranded DNAs, various chemical approaches for double-duplex invasion have been developed. In the previous research, a photo-induced double-duplex invasion (pDDI) is introduced that uses artificial nucleotides, 3-cyanovinylcarbazole nucleoside (^CNVK) as photo-crosslinker and 5-cyanouridine (^CU) as inter-probe photo-crosslinking inhibitor. In this study, while investigating the invasion mechanism of the pDDI, the invasion independence of pDDI probes is discovered and proposed a photo-induced duplex invasion (pDI) that achieves fast invasion using only ^CNVK while still maintaining a high invasion efficiency. This rapid pDI approach provides a powerful new tool for site-specific manipulation of genomic DNA.