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Related Experiment Video

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First Report on Biosynthesis of Riboflavin by Microbacterium proteolyticum BWBTDIPO1 - Extraction, Characterization

Indrani Paul1, Preeti Rajesh2, Subhajit Roy3

  • 1Department of Biotechnology, Brainware University, Kolkata, 700125, India. dip.bt@brainwareuniversity.ac.in.

Current Microbiology
|October 29, 2025
PubMed
Summary

A newly identified bacterial strain, Microbacterium proteolyticum, shows significant potential for Riboflavin (Vitamin B2) biosynthesis. This discovery offers a sustainable and cost-effective method for producing this vital nutraceutical.

Keywords:
BiosynthesisKEGG pathwayMetabolic pathwayMicrobacterium sp.Riboflavin

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Area of Science:

  • Microbiology
  • Biotechnology
  • Biochemistry

Background:

  • Sustainable and cost-effective production of nutraceuticals like Riboflavin is a significant challenge.
  • Bacterial strains capable of Riboflavin overproduction without genetic manipulation are rare.

Purpose of the Study:

  • To isolate and characterize a novel indigenous bacterial strain for Riboflavin biosynthesis.
  • To evaluate the growth kinetics and Riboflavin yield of the identified strain.
  • To elucidate the metabolic pathway and confirm the chemical identity of the produced Riboflavin.

Main Methods:

  • Isolation and identification of a gram-positive, rod-shaped bacterium as Microbacterium proteolyticum (GenBank Acc. No.: PQ517523).
  • Growth kinetics analysis in Luria Bertini broth.
  • Riboflavin yield quantification and production kinetics using the Luedeking-Piret model.
  • In-silico metabolic pathway analysis using KEGG and MetaCyc databases.
  • Chemical characterization of Riboflavin using UV-Vis spectroscopy, FTIR, and 1H NMR.

Main Results:

  • Microbacterium proteolyticum exhibited optimal growth and a maximum specific growth rate of 0.887 h⁻¹.
  • Achieved a high Riboflavin yield of 459.31 ± 22.79 mg/L (49.79 ± 1.29 mg/g biomass).
  • Luedeking-Piret model indicated mixed production characteristics (α=0.244, β=0.16) with a yield coefficient of 0.304.
  • In-silico analysis confirmed the involvement of the rib operon in Riboflavin production.
  • Spectroscopic and NMR analyses confirmed the extracted compound as Riboflavin.

Conclusions:

  • Microbacterium proteolyticum represents a novel and promising indigenous bacterial strain for sustainable Riboflavin biosynthesis.
  • The strain demonstrates high yield and mixed production kinetics, suitable for industrial applications.
  • This discovery has significant implications for the pharmaceutical, food, and cosmetic industries seeking natural Riboflavin sources.