Integrative Transcriptomic and Genomic Analysis Reveals the Regulatory Mechanisms Underlying Semen Quality Stability...

Area of Science:

• Animal Science
• Reproductive Biology
• Genetics

Background:

• Gander reproductive performance is significantly influenced by semen quality.
• Seasonal breeders, ganders often experience semen quality decline, with underlying mechanisms poorly understood.
• Maintaining stable semen quality is vital for waterfowl production efficiency.

Purpose of the Study:

• To systematically investigate gander semen quality parameters across different laying stages.
• To identify molecular mechanisms regulating semen quality stability in ganders.
• To provide references for genetic selection and reproductive management in waterfowl.

Main Methods:

• Semen quality assessment and histomorphological analysis of ganders at early, peak, and late laying stages.
• Transcriptomic and selective sweep analysis to identify key genes.
• Protein-protein interaction network construction and RT-qPCR validation.

Main Results:

• Ganders with stable semen quality exhibited superior parameters (e.g., sperm density) and larger testes compared to unstable groups.
• Four hub genes (EP300, RAB31, PRKG1, PIK3R1) and associated KEGG pathways (adherens junction, gap junction, etc.) were identified.
• Specific gene expression patterns (e.g., higher EP300, RAB31, PRKG1 in unstable; higher PIK3R1 in stable) were linked to semen quality stability.

Conclusions:

• Novel insights into molecular mechanisms of gander semen quality stability during the breeding season were provided.
• Gene expression patterns of EP300, RAB31, PRKG1, and PIK3R1 are associated with semen quality stability.
• Findings offer valuable references for genetic selection and reproductive management in waterfowl breeding.