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Updated: Jan 13, 2026
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Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
Published on: April 22, 2019
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Qualitative Changes in Collagen Induced by UV Irradiation Affect Its Interaction with Cells
Ayumi Asari1,2,3, Hiroaki Sakaue2, Touko Kumagai1
1Department of Biochemistry, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Biological & Pharmaceutical Bulletin
|October 29, 2025
Summary
UVB radiation damages collagen in the extracellular matrix (ECM), altering its structure and reducing cell interactions. This damage accelerates skin aging and increases susceptibility to degradation, revealing a new photoaging mechanism.
Area of Science:
- Biochemistry
- Dermatology
- Cell Biology
Background:
- The extracellular matrix (ECM), particularly collagen, is crucial for tissue structure and integrity.
- Aging and UV radiation (photoaging) decrease collagen, leading to skin aging signs like wrinkles.
- The impact of UVB on the ECM's interaction with cells remains incompletely understood.
Purpose of the Study:
- To investigate how UVB radiation alters the ECM, specifically collagen.
- To determine the effects of UVB-induced ECM changes on cell behavior and collagen degradation.
Main Methods:
- UVB irradiation of collagen.
- Analysis of collagen degradation and carbonylation using mass spectrometry (LC-MS/MS).
- Assessment of cell-collagen interactions (adhesion, proliferation, contraction).
Main Results:
- UVB irradiation caused collagen degradation and carbonylation, altering its structure.
- UVB-modified collagen showed reduced cell adhesion, proliferation, and contraction.
- Irradiated collagen was more vulnerable to matrix metalloproteinase-1 (MMP-1) degradation.
- Carbonylative modifications were identified on proline, arginine, and lysine residues.
Conclusions:
- UVB-induced collagen alterations in the ECM impact cellular functions.
- Carbonylative modifications disrupt collagen structure, affecting cell-matrix interactions.
- ECM modification is a key mechanism in photoaging, influencing skin cell behavior.
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