Innovation of Spectroscopic Approach for Investigation of Lurasidone in Pharmaceutical Formulations and Spiked Human...

A fast and economical flow injection-fluorometric technique has been employed for the estimation of lurasidone (LUR) in pharmaceutical formulations and biological fluids. LUR natively fluoresces due to the presence of the benzothiazole ring in its chemical composition. The assay relies on detecting the intense native fluorescence of LUR at 398 nm after excitation at 316 nm. Phosphate buffer (pH: 4.5, 10 mM): Acetonitrile (30:70 v/v) was used as the carrier solution, and the flow rate was 0.5 mL min^-1. Based on peak area, the calibration graph was linear throughout the range of 30-800 ng mL^-1 of LUR with a correlation coefficient (r) of 0.9999. The limit of quantitation was 21.7 ng mL^-1, while the limit of detection was 7.16 ng mL^-1. The approach was applied to quantify LUR in pharmaceuticals and human plasma. The approach was assessed in accordance with ICH specifications. There were no excipient-related interferences during the application of the analyses.