Related Experiment Video

Updated: Jan 12, 2026

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
06:32

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

Published on: September 1, 2010

16.8K

Global lncRNA expression profiles in medulloblastoma reveal crucial lncRNA-oncogene interactions in Sonic hedgehog

Iván Martínez de Estíbariz1, Unai Illarregi1, Daniel Sinnett2,3

  • 1Department of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa, Spain.

Neuro-Oncology Advances
|October 30, 2025
PubMed
Abstract

Insights

This study identifies specific long noncoding RNAs (lncRNAs) as potential biomarkers for medulloblastoma (MB) molecular groups. These lncRNAs, including Lnc-SMARCA2, MGC32805, and LOC107986446, are linked to specific MB subtypes and oncogenes, offering new therapeutic targets.

Area of Science:

  • Genomics
  • Molecular Biology
  • Oncology

Background:

  • Medulloblastoma (MB) characterization is improving with multi-omic studies, but novel molecular biomarkers are needed.
  • Current profiling focuses on the protein-coding genome, leaving noncoding regions unexplored.
  • Long noncoding RNAs (lncRNAs) are emerging as crucial regulators in MB and potential key biomarkers.

Purpose of the Study:

  • To identify lncRNAs specific to MB molecular groups.
  • To explore the role of lncRNAs in MB pathogenesis and disease mechanisms.
  • To enhance understanding of MB's genomic landscape through noncoding region analysis.

Main Methods:

  • RNA-sequencing (RNA-seq) data from 261 MB patients (54 Spanish, 207 public).
  • Expression analysis and Weighted Gene Coexpression Network (WGCNA) to identify lncRNA-oncogene interactions.
  • Examination of group-specific interactions to infer pathogenic roles.

Main Results:

  • LncRNA expression profiles identified 4 clusters corresponding to MB molecular groups.
  • Group-specific lncRNAs were found for Sonic hedgehog (SHH), Group 3 (Gr3), and Group 4 (Gr4) MB.
  • Lnc-SMARCA2 (SHH), MGC32805/LOC107986446 (Gr4), and a 5-lncRNA signature (Gr3) were identified and linked to specific oncogenes and pathways.

Conclusions:

  • Lnc-SMARCA2, MGC32805, and LOC107986446 are exclusively deregulated in SHH and Gr4 MB, respectively.
  • These lncRNAs are associated with group-specific MB oncogenes, indicating their role in pathogenesis.
  • These findings highlight promising novel biomarkers and therapeutic targets for MB treatment.
Keywords:
cancer biomarkersgene expression and interactionlong noncoding RNAsmedulloblastomatherapeutic targets

More Related Videos

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice05:10

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice

Published on: October 3, 2010

12.6K
Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

1.0K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.8K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
9.8K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.3K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
11.1K