Related Experiment Video
Updated: Jan 12, 2026

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans
Published on: August 16, 2017
A Global Transcriptomic Analysis Reveals Body Weight-Specific Molecular Responses to Chronic Orange Juice Consumption
Layanne Nascimento Fraga1, Dragan Milenkovic2, Isabella de Araújo Esteves Duarte1
1Food Research Center (FoRC) and School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
None:
Consumption of orange juice (OJ) has been linked to a lower incidence of cardiovascular diseases. However, molecular mechanisms underlying these health benefits remain unclear. This study aimed to investigate the molecular pathways involved in the health benefits of chronic OJ consumption through global transcriptomic approach in peripheral blood mononuclear cells (PBMCs). Healthy volunteers consumed 500 mL of OJ daily for 60 days. PBMCs were isolated from the blood, and the gene expression profiling was performed using Clariom microarrays. Genomic analysis revealed 1705 differentially expressed genes, including IL6, IL1β involved in inflammation, GSK3B, RIPK1 related to lipid metabolism, and NAMPT, NLRP3 involved in blood pressure regulation. Moreover, the expression of 66 miRNAs, 19 long-non-coding RNAs (lncRNAs), and 67 small nucleolar RNAs (snoRNAs) was modulated. Moreover, subgroup analysis revealed different transcriptomic responses between normal-weight (NW) and overweight (OW) individuals. Genes related to lipid metabolism and adipogenesis were differentially expressed only in OW, including GSK3B, GRK6, miR-548i, and miR-1292-3p. In contrast, genes related to the inflammation process, like STAT3, MAPK, and miRNAs (miR-1185-2-5p), were observed only in NW. These findings suggest that body weight may influence molecular response to bioactive compounds in OJ and provide information for personalized recommendations on the consumption of flavonoid-rich foods.
Related Concept Videos
Regulation of Food Intake
Regulation of Metabolism
Regulation of the Digestive System
The effectors in this regulation system are glands and smooth muscles. Activation of...
Hormonal Regulation of Blood Pressure
Epinephrine and Norepinephrine
The adrenal medulla releases epinephrine and norepinephrine, catecholamines that enhance and extend the sympathetic or "fight or flight" physiological response. These hormones escalate heart rate and the force of contraction...
Metabolic States of the Body: The Postabsorptive State
Initially, glycogen stored in the liver is broken down to release glucose into the bloodstream, while glycogen in the muscles is broken down to supply glucose for energy directly within the muscle cells. As glycogen stores diminish,...
Metabolic States of the Body: Fasting and Starvation

