Related Experiment Video

Updated: Jan 12, 2026

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans
09:23

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Published on: August 16, 2017

8.6K

A Global Transcriptomic Analysis Reveals Body Weight-Specific Molecular Responses to Chronic Orange Juice Consumption

Layanne Nascimento Fraga1, Dragan Milenkovic2, Isabella de Araújo Esteves Duarte1

  • 1Food Research Center (FoRC) and School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.

Molecular Nutrition & Food Research
|October 31, 2025
PubMed

Insights

Daily orange juice (OJ) consumption impacts gene expression in blood cells, influencing inflammation and metabolism. These effects differ between normal-weight and overweight individuals, suggesting personalized dietary advice.

Area of Science:

  • Nutritional Immunology
  • Molecular Biology
  • Genomics

Background:

  • Orange juice (OJ) consumption is associated with reduced cardiovascular disease risk.
  • The precise molecular mechanisms behind OJ's cardiovascular benefits are not fully understood.

Purpose of the Study:

  • To explore the molecular pathways influenced by chronic OJ intake.
  • To investigate gene expression changes in peripheral blood mononuclear cells (PBMCs) using a transcriptomic approach.

Main Methods:

  • Healthy volunteers consumed 500 mL of OJ daily for 60 days.
  • Gene expression profiling of PBMCs was conducted using Clariom microarrays.
  • Bioinformatic analysis identified differentially expressed genes, miRNAs, lncRNAs, and snoRNAs.

Main Results:

  • 1705 genes were differentially expressed, including those involved in inflammation (IL6, IL1β), lipid metabolism (GSK3B, RIPK1), and blood pressure regulation (NAMPT, NLRP3).
  • Expression of 66 miRNAs, 19 lncRNAs, and 67 snoRNAs was modulated.
  • Overweight individuals showed distinct gene expression changes related to lipid metabolism and adipogenesis, while normal-weight individuals exhibited changes in inflammation-related genes.

Conclusions:

  • Chronic OJ consumption significantly alters gene expression in PBMCs.
  • Body weight influences the molecular response to OJ, impacting pathways differently in normal-weight versus overweight individuals.
  • Findings support personalized nutrition strategies for flavonoid-rich food consumption.
Keywords:
blood pressurebody mass indexflavanonesinflammationlipid metabolismorange juicetranscriptomic

More Related Videos

The TreadWheel: Interval Training Protocol for Gently Induced Exercise in Drosophila melanogaster07:21

The TreadWheel: Interval Training Protocol for Gently Induced Exercise in Drosophila melanogaster

Published on: June 8, 2018

12.1K
Palatable Western-style Cafeteria Diet as a Reliable Method for Modeling Diet-induced Obesity in Rodents09:10

Palatable Western-style Cafeteria Diet as a Reliable Method for Modeling Diet-induced Obesity in Rodents

Published on: November 1, 2019

11.4K

Related Concept Videos

Regulation of Food Intake01:30

Regulation of Food Intake

Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...
2.3K
Regulation of Metabolism01:19

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
11.4K
Regulation of the Digestive System01:25

Regulation of the Digestive System

Digestive activity regulation hinges on three primary components. Activation is prompted by a multitude of mechanical and chemical indicators, primarily detected by receptors within the stomach and intestines' walls. These receptors predominantly respond to factors such as mechanical stretching of the organ walls, changes in pH and osmolarity, and the presence of digesting materials and their by-products.
The effectors in this regulation system are glands and smooth muscles. Activation of...
2.9K
Hormonal Regulation of Blood Pressure01:17

Hormonal Regulation of Blood Pressure

Endocrinal or hormonal intervention in the cardiovascular system is predominantly exerted by the catecholamines - epinephrine and norepinephrine, as well as a slew of hormones that interact with renal function to modulate blood volume.
Epinephrine and Norepinephrine
The adrenal medulla releases epinephrine and norepinephrine, catecholamines that enhance and extend the sympathetic or "fight or flight" physiological response. These hormones escalate heart rate and the force of contraction...
5.8K
Metabolic States of the Body: The Postabsorptive State01:18

Metabolic States of the Body: The Postabsorptive State

The postabsorptive state usually starts about four hours after a meal and lasts until the next meal is eaten. During this time, the digestive system stops absorbing nutrients, and the body uses stored energy reserves to maintain stable blood glucose levels.
Initially, glycogen stored in the liver is broken down to release glucose into the bloodstream, while glycogen in the muscles is broken down to supply glucose for energy directly within the muscle cells. As glycogen stores diminish,...
1.2K
Metabolic States of the Body: Fasting and Starvation01:24

Metabolic States of the Body: Fasting and Starvation

During the initial hours of fasting, the body uses up its glycogen stores as an energy source. Once these glycogen reserves are depleted, the body begins breaking down stored triglycerides and structural proteins. During this stage, glycerol becomes a key substrate for gluconeogenesis, while free fatty acids undergo beta-oxidation to provide energy for tissues, such as skeletal muscle. In the fasting state, the body spares protein breakdown as much as possible to conserve muscle and structural...
2.7K