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Updated: Jul 24, 2026
An Improved and High Throughput Respiratory Syncytial Virus RSV Micro-neutralization Assay
Published on: January 26, 2019
Evaluation of Measles Vaccine Immunogenicity and Durability Using A Pseudotyped Virus Neutralization Assay
1Division of HIV/AIDS and Sexually Transmitted Virus Vaccines, Institute for Biological Product Control, State Key Laboratory of Drug Regulatory Science, National Institutes for Food and Drug Control (NIFDC), Beijing, China.
Introduction:
This study aimed to establish a robust method for monitoring measles vaccine-induced immunity and assessing population-level serostatus.
Methods:
This study constructed a vesicular stomatitis virus (VSV)-based pseudotyped virus system expressing envelope proteins from seven major circulating measles genotypes (H1, B3, D4, D8, D9, D11, G3) and the Schwarz vaccine strain (genotype A), thereby enabling a high-throughput neutralization assay for antibody detection.
Results:
Vaccination induced a substantial increase in neutralizing antibody geometric mean titers (GMT) post-immunization (4,808 after the first dose; 5,326 after the second dose), with antibody levels remaining elevated in 4-year-old children (GMT: 3,834). Cross-neutralization activity against different genotypes varied by less than 6.4-fold, demonstrating broad protective immunity. However, 12% of adult sera tested were seronegative, revealing the presence of susceptible populations.
Conclusions:
This study confirms the robust immunogenicity of the current measles vaccine and establishes a valuable tool for serosurveillance and long-term immunity assessment.
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