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Cycloartane-type saponins from Curculigo orchioides and their anti-inflammatory activity
Le Thanh Huong1, Vu Duong Bach1, Pham The Hai1
1University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology (VAST) 18 Hoang Quoc Viet Hanoi Vietnam nguyen-hai.dang@usth.edu.vn.
Abstract:
Curculigo orchioides is a traditional medicinal plant used in Asia for treating inflammation and related ailments. In this study, nine compounds were isolated, including six novel cycloartane-type saponins, namely curculigosaponins P-U (1-6) and three known compounds. Among the six new compounds, compound 1 exhibited moderate inhibition of NO production (IC50 = 37.21 μM) in LPS-stimulated RAW 264.7 macrophages. It also suppressed the expression of TNF-α and IL-6, and downregulated iNOS and COX-2 protein levels, confirming its anti-inflammatory activity. Molecular docking analysis revealed favorable binding interactions of compound 1 with key inflammatory targets (iNOS, IL-6, TNF-α, and COX-2), supporting its proposed mechanism of action. These results suggest that compound 1 (curculigosaponins P) is a promising anti-inflammatory lead compound from C. orchioides and deserves further investigation.
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