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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jan 12, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

DNA self-assembly-based direct fluorescence encoding of targets enables simultaneous 10-plex microRNA detection.

Xiaozhi Huang¹, Lingpeng Lu², Yilin Yan¹

¹Department of Clinical Laboratory Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, PR China.

Biosensors & Bioelectronics

|October 31, 2025

Summary

This summary is machine-generated.

This study introduces a novel imaging system for detecting microRNAs (miRNAs), crucial biomarkers for disease. The new method enhances sensitivity and allows for multiplexed miRNA detection, improving early tumor screening and precision medicine.

Keywords:

DNA assembly Fluorescence encoding system MicroRNA Multiplex detection

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

Biochemistry
Molecular Biology
Biotechnology

Background:

MicroRNAs (miRNAs) are key post-transcriptional regulators and valuable biomarkers for disease diagnosis and precision medicine.
Existing miRNA detection technologies, such as Luminex xMAP, face limitations in sensitivity and reproducibility.

Purpose of the Study:

To develop an innovative imaging-based detection system for microRNAs.
To overcome the limitations of current miRNA detection methods by enhancing sensitivity and enabling multiplexed detection.

Main Methods:

Leveraged DNA self-assembly and fluorescence barcoding technologies for a novel detection system.
Utilized programmable DNA assemblies for specific target recognition and signal amplification.
Employed magnetic bead-mediated target enrichment and a four-color fluorescent encoding system for multiplexed detection of 10 miRNAs.

Main Results:

The system demonstrated enhanced sensitivity through magnetic bead-mediated target enrichment and DNA self-assembly.
A four-color fluorescent encoding system enabled multiplexed detection of 10 miRNAs in a single tube.
Validation in MCF-7 breast cancer cell lines confirmed robust performance in complex biological matrices.

Conclusions:

The developed platform offers a streamlined, cost-effective, and reproducible method for miRNA detection.
This innovative system holds potential for early tumor screening and advancing precision medicine.
The system provides a new technical means for sensitive and multiplexed miRNA analysis.