Light-triggered electrochemical biosensor using singlet oxygen for self-powered operation and glucose detection.

Area of Science:

• Electrochemistry
• Biosensing
• Photochemistry

Background:

• Photosensitization generates reactive oxygen species.
• Electrochemical detection offers sensitive analyte quantification.
• Glucose oxidase is a key enzyme for glucose sensing.

Purpose of the Study:

• To develop a light-activated glucose biosensor.
• To integrate photosensitization with electrochemical detection.
• To achieve tunable, on-demand signal control for biosensing.

Main Methods:

• Utilizing Eosin Y as a photosensitizer to generate singlet oxygen (¹O₂).
• Immobilizing Eosin Y on a carbon electrode within a polymer matrix.
• Coupling the ¹O₂-mediated process with glucose oxidase (GOx) for glucose detection.
• Employing green light (520 nm) illumination for sensor activation.

Main Results:

• The biosensor demonstrated a measurable current initiated by light-induced ¹O₂.
• Glucose addition reversed current flow due to GOx competition for electrons.
• A sensitive correlation between current magnitude and glucose concentration was observed.
• The biosensor achieved a detection limit (LOD) of 2.8 mM for glucose (0-73 mM range).

Conclusions:

• A novel light-activated sensing platform was successfully developed.
• The platform enables tunable, on-demand signal control using light and ¹O₂.
• This approach offers a new strategy for adaptive, real-time biosensing technologies.