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Published on: August 25, 2018
OsTIP1;2 is a key regulator of rice seed germination and stress resilience
Xin Liu1, Linli Ben2, Siyuan Tang2
1Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, Jiangsu, China; Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, Jiangsu, China; Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
Abstract:
Uniform and rapid germination of rice seeds is essential for successful direct seeding cultivation. In this study, we show that the tonoplast aquaporin OsTIP1;2 must be maintained within a narrow expression window to ensure optimal germination. Both loss- and gain-of-function lines exhibit reduced water uptake and delayed germination, accompanied by disturbed (abscisic acid) ABA/ (gibberellins) GA homeostasis and decreased α-amylase activity. Transcriptome profiling indicates associations with hormone signaling and starch catabolism. Moreover, altered expression of OsTIP1;2 results in differing sensitivities of germinating seeds to salt stress, impacting germination rates under saline conditions. Haplotype analysis revealed that rice varieties carrying Haplotype 1 (Hap1) allele of OsTIP1;2 are associated with improved germination under salt stress.
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