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Scalable Bioelectronic Production of Extracellular Vesicles for Cardiac Tissue Regeneration
Lingyuan Meng1, Jing Zhang2, Pengju Li1
1Pritzker School of Molecular Engineering, University of Chicago, Chicago, IL 60637, USA.
Device
|November 3, 2025
Summary
Bioelectronic stimulation enhances extracellular vesicle (EV) production and quality. Electrically-induced EVs (e-EVs) show improved therapeutic potential for cardiac repair, offering a scalable solution for regenerative medicine.
Area of Science:
- Biotechnology
- Regenerative Medicine
- Bioelectronics
Background:
- Extracellular vesicle (EV) therapies face challenges with low production efficiency and scalability.
- Current methods to boost EV yield often degrade vesicle quality and cell health.
Purpose of the Study:
- To develop a bioelectronic platform for enhanced EV production.
- To assess the therapeutic potential of electrically-induced EVs (e-EVs) in cardiac repair.
Main Methods:
- A bioelectronic platform with interdigitated electrodes for low-voltage, low-frequency biphasic stimulation.
- Simultaneous super-resolution imaging of EV biogenesis using TIRF microscopy.
- Application of the platform to primary cardiac cells and a murine myocardial infarction model.
Main Results:
- Bioelectrical stimulation significantly increased EV secretion from cardiac cells without compromising viability.
- Electrically-induced EVs (e-EVs) demonstrated enhanced microRNA loading, preserved tissue tropism, and therapeutic efficacy.
- Treatment with miRNA-loaded e-EVs improved cardiac function and reduced fibrosis in a murine myocardial infarction model.
Conclusions:
- Bioelectronic modulation is a promising, non-destructive strategy for scalable EV production.
- Electrically-induced EVs (e-EVs) offer enhanced functional performance for regenerative medicine applications.
- This approach holds potential for advancing EV-based therapies in clinical settings.
