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The Use of B-cell Tetramers for the Study of Trypanosoma cruzi-Specific B cells

Damián Pérez-Mazliah1

  • 1Hull York Medical School and York Biomedical Research Institute, University of York, York, UK. damian.perezmazliah@york.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|November 3, 2025
PubMed

Tracking, isolating, and studying antigen-specific B cells is challenging due to their low frequencies. Here, I describe a protocol to study enriched Trypanosoma cruzi (T. cruzi)-specific B cells by flow cytometry. Compared to alternative strategies (e.g., ELISA and ELISPOT), this approach allows detailed phenotyping and/or cell sorting for downstream analysis of T. cruzi-specific B cells.

Keywords:
Antigen-specific B cellsB-cell tetramersFlow cytometryFluorochrome-conjugated tetrameric antigen probesMagnetic enrichmentParasite-specific antibody responsesT. cruzi trans-sialidaseT. cruzi-specific B-cell responses

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