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Related Concept Videos

Enzyme-linked Receptors01:00

Enzyme-linked Receptors

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Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
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Introduction to Enzymes01:22

Introduction to Enzymes

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The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that...
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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Enzyme Kinetics01:19

Enzyme Kinetics

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Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
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Enzyme Inhibition01:30

Enzyme Inhibition

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Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
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Enzymes02:34

Enzymes

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Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
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Related Experiment Video

Updated: Jan 12, 2026

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
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Open Enzyme Database: a community-wide repository for sharing enzyme data.

Le Yuan1,2,3, David M Bianchi3,4,5, Katherine Arneson3,4,5

  • 1Department of Chemical and Biomolecular Engineering, University of Illinois Urbana-Champaign, Urbana, IL 61801, United States.

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The Open Enzyme Database (OED) offers a centralized, AI-enhanced platform for enzyme data. This resource aims to accelerate biocatalysis, synthetic biology, and AI-driven enzyme discovery through improved data sharing and prediction tools.

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Area of Science:

  • Biochemistry
  • Bioinformatics
  • Computational Biology

Background:

  • Enzymes are crucial biological catalysts with diverse applications.
  • Existing enzyme databases suffer from infrequent updates, lack of standardization, and limited machine learning support.
  • There is a need for a comprehensive, up-to-date, and machine learning-ready enzyme repository.

Purpose of the Study:

  • To develop the Open Enzyme Database (OED), a community-driven repository for enzyme data.
  • To create a user-friendly web-based infrastructure for sharing and exploring enzyme information.
  • To integrate advanced AI and cheminformatics tools for enzyme property prediction and discovery.

Main Methods:

  • Development of a community-wide repository for enzyme data.
  • Curation of enzyme kinetic parameters, structural data, reactions, assay conditions, and functional annotations.
  • Implementation of artificial intelligence (AI) and cheminformatics algorithms for enzyme property prediction and identification.

Main Results:

  • The first release of the OED provides curated data on enzyme kinetics, structures, reactions, and annotations.
  • The database incorporates AI tools for predicting enzyme properties.
  • Cheminformatics algorithms are included for identifying promising enzymes.
  • The OED is accessible via a web platform.

Conclusions:

  • The Open Enzyme Database (OED) provides a valuable resource for the scientific community.
  • The OED is expected to accelerate research in biocatalysis, systems biology, synthetic biology, and enzyme engineering.
  • The integration of AI tools within the OED will advance enzyme discovery and application development.